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Journal of The Korean Society of Emergency Medicine 2010;21(4): 437-443. |
| Effects of H2O2 on Cardiac Rate and Apoptosis of Primary Rat Cardiomyoblasts in in Vitro Culture Systems |
| Tae Eun Cho, Yong Jae Han, Yong Hwang, Su Jin Yoo |
| Department of Emergency Medicine, Wonkwang University College of Medicine, Iksan, Korea. ysoojin@wmc.wonkwang.ac.kr |
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| ABSTRACT |
PURPOSE:
This work was intended to establish experimental conditions for monitoring the effect of ischemic/reperfusion injury on the beating capability of and apoptotic damage to primary rat cardiomyoblasts.
METHODS:
In an in vitro system, cardiac rate differed depending on the number of days after birth that the cells were isolated. We maintained a mean rate of 62 times per min until 4 or 5 days in culture. To generate ischemic/reperfusion injury, primary rat cardiomyoblasts were treated with hydrogen peroxide (H2O2).
RESULTS:
Treatment with H2O2 significantly decreased the cardiac rate of primary rat cardiomyoblasts in a time- and dose-dependent manner. Interestingly, the cardiac rate of primary rat cardiomyoblasts abruptly dropped prior to the decrease in cell viability. H2O2 also induced a decrease in the expression of heme oxygenase-1 (HO-1) protein in P2 primary rat cardiomyoblasts in a time-dependent manner.
Moreover, treatment with H2O2 resulted in an increase in the proportion of cells in the sub-G0/G1 phase, indicating that H2O2 induces the apoptotic death of P2 primary rat cardiomyoblasts. However, the intracellular level of calcium was markedly decreased under the same experimental conditions.
CONCLUSION:
An in vitro culture system is useful for investigating the mechanism underlying the beating capability of rat heart cells and the mechanism underlying apoptotic damage to primary rat cardiomyoblasts induced by ischemic/reperfusion injury, including ROS-induced damage. |
| Key words:
Hydrogen peroxide, Apoptosis, Heme oxygenase 1, Ischemia |
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