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Journal of The Korean Society of Emergency Medicine 1996;7(4): 466-481. |
| THE EXPERIMENTAL STUDY FOR THE EFFECT OF HIGH DOSAGE VITAMIN一C THERAPYIN RAT MODEL OF SEPSIS SYNDROME INDUCED BY PANPERITONITIS |
| Joong Eui Rhee1, Yeon Kwon Jeong2, Sung Eun Jung3 |
1Department of Emergency Medicine, Seoul National University Hospital
2Department of Emergency Medicine, Sam-Sung Medical Center
3Department of Surgery, Seoul National University Hospital |
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Published online: December 31, 1996. |
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| ABSTRACT |
1. Background
In sepsis syndrome, peroxidation reaction of polyunsaturated cell-membrane lipids by reactive oxygen free radicals is known as the main mechanism of cell injury. Recent explosive rush of studies on the biologic functions of NO has made it clear that NO is essential to the vessel function and microcirculation and increased production of NO in sepsis has important pathophysiologic actions.
2. Purpose
: This study is designed to evaluate the therapeutic effects of vitamin-C, a famous antioxidant, on the liver tissue protection in severe intraperitoneal infection.
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Method
: Female rats of Sprague-Dawley as experiment animal were divided into a control group(group-A) and two experimental groups of a peritonitis group (group-B) and a peritonitis and-vitamin-C group(group-C). Peritonitis had been induced by cecal ligation and perforation under ketamine anesthesia. Vitamin-C (600 mg/kg) was injected intramuscularly. As a index of peroxidation reaction, malonclialdehyde (MDA)level and catalase activity of the liver tissue were examined. And NOS activity in liver tissue was measured. In blood, the concentration of TNF- α was measured with ELISA method.
4. Results : On microscopic examination, liver tissue of group-B showed lymphocyte and neutrophil infiltration, fatty degeneration of hepatocyte, and destruction of liver lobular structure. In group-C, there were lesser lymphocyte and neutrophil infiltration and lesser structural injury in the liver tissue in comparison with in group-B. MDA levels of liver tissue were remarkably increased in group-B.
Catalase activity of group-B was used up in very early phase of peritonitis. In comparison with group-B, group-C had significantly lower MDA levels and significantly higher catalase activity. Plasma TNF- α levels of group-B were markedly increased compared with group-A. Group-C showed markedly suppressed plasma TNF- α levels, compared to group-B. NOS activity in liver tissue of group-B was significantly increased. Vitamin-C suppressed increment of NOS activity.
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Conclusion
Cell injury by reactive oxygen free radicals was thought to play a key role in sepsis. High dosage of vitamin-C had therapeutic cell-protective effect against lipid peroxidation, suppressive action on the TNF- α production, and inhibitory effect on NOS activation. The therapeutic effect of vitamin-C in sepsis was supposed to be mediated mainly by their antioxidant function. But the exact interaction between peroxidation reaction and NO or TNF- α could not elucidated clearly. Further well-designed experiment is necessary.
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| Key words:
Sepsis syndrome, Peroxidation reaction, Vitamin-C, TNF- α , Nitric oxide |
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