Changes in Histopathology and Tumor Necrosis Factor-<i>α</i> Levels in the Hearts of Rats Following Asphyxial Cardiac Arrest

Lee, Jung Hoon; Lee, Tae-Kyeong; Kim, In Hye; Lee, Jae Chul; Won, Moo-Ho; Park, Joon Ha; Ahn, Ji Hyeon; Shin, Myoung Chul; Ohk, Taek Geun; Moon, Joong Bum; Cho, Jun Hwi; Park, Chan Woo; Tae, Hyun-Jin

J Korean Soc Emerg Med > Volume 28(5); 2017 > Article

Original Article

Journal of The Korean Society of Emergency Medicine 2017;28(5): 449-456.

Changes in Histopathology and Tumor Necrosis Factor-α Levels in the Hearts of Rats Following Asphyxial Cardiac Arrest

Jung Hoon Lee¹, Tae-Kyeong Lee², In Hye Kim², Jae Chul Lee², Moo-Ho Won², Joon Ha Park³, Ji Hyeon Ahn³, Myoung Chul Shin¹, Taek Geun Ohk¹, Joong Bum Moon¹, Jun Hwi Cho¹, Chan Woo Park¹, Hyun-Jin Tae⁴

¹Department of Emergency Medicine, Kangwon National University Hospital, Kangwon National University School of Medicine, Chuncheon, Korea
²Department of Neurobiology, Kangwon National University School of Medicine, Chuncheon, Korea
³Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon, Korea
⁴Bio Safety Research Institute, Chonbuk National University College of Veterinary Medicine, Iksan, Korea

Correspondence

Chan Woo Park ,Tel: 033-258-2292, Fax: 033-258-2451, Email: bonaeboa@naver.com,
Hyun-Jin Tae ,Tel: 063-850-0957, Fax: 063-850-0910, Email: hjtae@jbnu.ac.kr,

Received: May 8, 2017; Revised: May 16, 2017 Accepted: June 14, 2017. Published online: October 31, 2017.

ABSTRACT

Purpose:
Post cardiac arrest (CA) syndrome is associated with a low survival rate in patients who initially have a return of spontaneous circulation (ROSC) after the CA. The aim of this study was to examine the histopathology and inflammatory response in the heart during post CA syndrome.
Method:
Asphyxial CA was induced in male Sprague-Dawley rats and the survival rate of the rats was determined during the post resuscitation phase.
Results:
Survival of the rats decreased after CA: 66.7% at 6 hours, 36.7% at 1 day, and 6.7% at 2 days after the ROSC following CA. The rats were sacrificed at 6 hours, 12 hours, 1 day, and 2 days after the ROSC, and their heart tissues were examined. Histopathological scores increased at 12 hours post CA. Afterwards, the histopathological changes were not significant. In addition, the levels of tumor necrosis factor-α immunoreactivity increased gradually after CA.
Conclusion:
The survival rate of the rats 2 days post CA was very low, even though the histopathological and inflammatory changes in the heart were not pronounced in the early stages following the CA.

Key words: Cardiac arrest, Heart, TNF-alpha