| Home | E-Submission | Sitemap | Contact Us |  
J Korean Soc Emerg Med > Volume 16(2); 2005 > Article
Journal of The Korean Society of Emergency Medicine 2005;16(2): 292-297.
Effects of Humoral Factors Released from Ischemic-preconditioned Hearts on Survival of Pancreatic Cells Exposed to Hypoxia: An In vitro Study Using Neonatal Pigs
Won Jae Lee, Se Kyung Kim, Yo Sub Park
1Department of Emergency Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea. arflee@hanmail.net
2Department of Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Brief myocardial ischemia evokes a cardioprotective response, referred to as "Ischemic Preconditioning", that limits injury caused by a subsequent prolonged ischemic insult. The myocardial ischemic preconditioning effect can be induced by ischemia of "distant" cardiac and noncardiac tissue, implicating the involvement of an as-yet unidentified humoral trigger. The purpose of this study was to prove the protective effect of a preconditioning ischemic trigger (PIT) obtained from coronary effluent to isolated pancreatic cells under hypoxic condition in neonatal pigs.
Isolated hearts were preconditioned 5 times with 5-min ischemia following 10-min reperfusion. Coronary effluent was collected during reperfusion, filtered by using a Sep-Pak C-18 catridge, and lyophilized after dissolving it with acetonitrile. Isolated pancreatic cells were divided into a PIT-treatment group and a control group, and each group was further divided into time-dependent and dose-dependent groups. Time-dependent groups were incubated under a hypoxic condition for durations of 1, 2, 3, and 4 hrs, and dose-dependent groups were treated with 3 different doses of PIT that had undergone hypoxic incubation for 4 hrs. Viability of the pancreatic cells after the hypoxic incubation period was evaluated by using a confocal microscope.
In the control group, the average viability of pancreatic cells after 4 hrs of hypoxia was 60.48 +/- 1.24%, and in the PIT-treated group, the value was 71.88 +/- 1.33%, the difference in the viability between the PIT-treated group and the control group after 4 hrs of hypoxia was statistically significant. In the dose-dependent groups, the viability of pancreatic cells was significantly larger in the groups treated with original PIT and 1/10 PIT than in the control group.
These data suggest that in the In-vitro pig model, PIT obtained from heart evoked ischemic tolerance to isolated pancreatic cells.
Key words: Ischemic preconditioning, Tolerance, Trigger
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
Related article
Editorial Office
The Korean Society of Emergency Medicine
TEL: +82-62-226-1780   FAX: +82-62-224-3501   E-mail: dlskdud416@naver.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © The Korean Society of Emergency Medicine.                 Developed in M2PI