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J Korean Soc Emerg Med > Volume 28(5); 2017 > Article
Journal of The Korean Society of Emergency Medicine 2017;28(5): 457-466.
C57BL/6 쥐 외상성 뇌손상 모델에서 뇌 손상 정도에 따른 조직병리학적 변화 및 신경행동학적 특징
오기영, 최동원, 장문순, 이지한, 김상철, 박정수, 이석우, 김 훈
충북대학교 의과대학 응급의학교실
Histopathological and Neurobehavioral Characterization in Adult Mice Exposed to Traumatic Brain Injury
Ki Young Oh, Dong Won Choi, Moon Soon Jang, Ji Han Lee, Sang Chul Kim, Jung Soo Park, Suk Woo Lee, Hoon Kim
Department of Emergency Medicine, Chungbuk National University College of Medicine, Cheongju, Korea
Correspondence  Hoon Kim ,Tel: 043-261-2847, Fax: 043-269-7810, Email: nichekh2000@chungbuk.ac.kr,
Received: July 26, 2017; Revised: July 27, 2017   Accepted: September 4, 2017.  Published online: October 31, 2017.
ABSTRACT
Purpose:
Traumatic brain injury (TBI) is a significant cause of morbidity and mortality worldwide. Severity of the initial insult is one of the most significant factors affecting outcome following TBI. In order to investigate the mechanisms of cellular injury and develop novel therapeutic strategies for TBI, we designed a standardized animal TBI model and evaluated histological and functional outcomes according to the degree of impact severity.
Method:
Male adult C57Bl/6 mice underwent controlled cortical impact (CCI) at varying depths of deflection (1.0-2.0 mm). We performed hematoxylin and eosin staining at 7 days after recovery from TBI. Neurobehavioral characterization after TBI was analyzed by the Barnes maze test, passive avoidance test, open field test, rotarod test, tail suspension test, and light/dark test.
Results:
We observed a graded injury response according to the degree of deflection depths tested (diameter, 3 mm; velocity, 3 m/s; and duration, 500 ms) compared to sham controls. In the Barnes maze test, the severe TBI (2 mm depth) group showed reduced spatial memory as compared with the sham and mild TBI (1 mm depth) groups at 7 days after TBI. There was a significant difference in the results of the open field test and light/dark test among the three groups.
Conclusion:
Our findings demonstrate that the graded injury responses following TBI resulted in differential histopathological and behavioral outcomes in a mouse experimental CCI model. Thus, a model of CCI with histologic/behavioral outcome analysis may offer a reliable and convenient design for preclinical TBI research involving mice.
Key words: Brain injuries, Mice, Neurobehavioral manifestations
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